U2af1^tm2.1Hev
Targeted Allele Detail

Summary

• Symbol: U2af1^tm2.1Hev
• Name: U2 small nuclear ribonucleoprotein auxiliary factor (U2AF) 1; targeted mutation 2.1, Harold E Varmus
• MGI ID: MGI:6273478
• Synonyms: IES34F
• Gene: U2af1 Location: Chr17:31866055-31877866 bp, - strand Genetic Position: Chr17, 17.02 cM
• Alliance: U2af1^tm2.1Hev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267082
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting vector contains a loxP site upstream of exon 2, a FRT-flanked neomycin selection cassette downstream of exon 2 followed by a loxP 2272 sequence, an inverted mutant exon 2 carrying the serine to phenylalanine (S34F) missense mutation, a loxP site and another loxP2272 sequence. Flp-mediated recombination removed the neomycin cassette. The mutant exon 2 replaces the wild-type exon after cre-mediated recombination. (J:267082)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any U2af1 Mutation: 32 strains or lines available

Notes

ES cell line = HG3 (129S6/SvEvTac x C57BL/6-Tg(UBC-GFP)30Scha/J)F1

References

• Original: J:267082 Fei DL, et al., Impaired hematopoiesis and leukemia development in mice with a conditional knock-in allele of a mutant splicing factor gene U2af1. Proc Natl Acad Sci U S A. 2018 Oct 30;115(44):E10437-E10446
• All: 1 reference(s)