Phf20l1^em1(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Phf20l1^em1(IMPC)Tcp
• Name: PHD finger protein 20-like 1; endonuclease-mediated mutation 1, The Centre for Phenogenomics
• MGI ID: MGI:6273283
• Gene: Phf20l1 Location: Chr15:66449409-66519825 bp, + strand Genetic Position: Chr15, 29.3 cM
• Alliance: Phf20l1^em1(IMPC)Tcp page
• IMPC: Phf20l1 gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele from project TCPR1219 was generated at The Centre for Phenogenomics by electroporating Cas9 ribonucleoprotein complexes with single guide RNAs having spacer sequences of ACGTGGCTGACTATCATCAT targeting the 5' side and AGTCAGTCACCTATTGTGCT targeting the 3' side of a critical exon. This resulted in a 476-bp del Chr15:66603886 to 66604361_insATAGTC resulting in a frameshift mutation in all annotated full length protein-coding transcripts.(GRCm38). (J:265051)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Phf20l1 Mutation: 52 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 2 reference(s)