Ghrl^em3Jmz
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ghrl^em3Jmz
• Name: ghrelin; endonuclease-mediated mutation 3, Jeffrey M Zigman
• MGI ID: MGI:6273205
• Gene: Ghrl Location: Chr6:113693080-113696841 bp, - strand Genetic Position: Chr6, 52.84 cM, cytoband E3
• Alliance: Ghrl^em3Jmz page

Mutation origin

• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Insertion
• Mutation details: An EcoRI site, 3 sequential in-frame stop codons and additional out of frame stop codons were introduced into exon 2 via CRISPR-cas9. Two founders, GKO1 and GKO3, were developed into lines. Immunohistochemical analysis confirmed the lack of expression in stomach sections from homozygous mice. (J:268617)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ghrl Mutation: 12 strains or lines available

References

• Original: J:268617 Rodriguez JA, et al., Ghrelin Receptor Agonist Rescues Excess Neonatal Mortality in a Prader-Willi Syndrome Mouse Model. Endocrinology. 2018 Dec 1;159(12):4006-4022
• All: 1 reference(s)