Col1a1^{tm1(tetO-GFP/RNAi:Sod2)Gsha}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1(tetO-GFP/RNAi:Sod2)Gsha}
• Name: collagen, type I, alpha 1; targeted mutation 1, Gerald Shadel
• MGI ID: MGI:6270374
• Synonyms: iSOD2 KD
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1(tetO-GFP/RNAi:Sod2)Gsha} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:267914
• Parent Cell Line: KH2 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: A replacement vector (TRE-tGFP-miRE\Sod2_835_shRNA_1) was constructed to have an FRT site, a Tet-responsive element followed by the constitutively active, minimal human cytomegalovirus promoter, an IRES-turboGFP sequence (tGFP; a fast-maturating and short-half-life green fluorescent protein variant); Sod2_835_shRNA_1 (placed in the tGFP 3' UTR), an RBG polyA sequence and an FRT site. The Sod2_835_shRNA_1 is comprised of a 141 bp shRNA sequence that yields significant reduction of SOD2 expression at the mRNA and protein level. The vector is inserted in the Col1a1 locus. (J:267914)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 161 strains or lines available

References

• Original: J:267914 Cox CS, et al., Mitohormesis in Mice via Sustained Basal Activation of Mitochondrial and Antioxidant Signaling. Cell Metab. 2018 Nov 6;28(5):776-786.e5
• All: 1 reference(s)