Tmem165^tm1.1Cnhm
Targeted Allele Detail

Summary

• Symbol: Tmem165^tm1.1Cnhm
• Name: transmembrane protein 165; targeted mutation 1.1, Kyle Cunningham
• MGI ID: MGI:6267063
• Synonyms: TMEM165^delta
• Gene: Tmem165 Location: Chr5:76331727-76357091 bp, + strand Genetic Position: Chr5, 40.56 cM
• Alliance: Tmem165^tm1.1Cnhm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276293
• Parent Cell Line: MC1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 76346717 of Chromosome 5 upstream of exon 2 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. (J:276293)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmem165 Mutation: 10 strains or lines available

References

• Original: J:276293 Snyder NA, et al., Milk biosynthesis requires the Golgi cation exchanger TMEM165. J Biol Chem. 2019 Mar 1;294(9):3181-3191
• All: 1 reference(s)