Polr2b^em1(IMPC)Bay
Endonuclease-mediated Allele Detail

Summary

• Symbol: Polr2b^em1(IMPC)Bay
• Name: polymerase (RNA) II (DNA directed) polypeptide B; endonuclease-mediated mutation 1, Baylor College of Medicine
• MGI ID: MGI:6266826
• Synonyms: Polr2b^-
• Gene: Polr2b Location: Chr5:77458331-77497175 bp, + strand Genetic Position: Chr5, 41.66 cM
• Alliance: Polr2b^em1(IMPC)Bay page
• IMPC: Polr2b gene page

Polr2b^em1(IMPC)Bay/Polr2b^em1(IMPC)Bay mice exhibit embryonic lethality, with embryos recovered at E3.5 but not at E7.5. Embryos at E3.5 appear as dying morulae. Mutants fail to hatch from the zona pellucida and and die after 3 days in culture.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6N
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at Baylor College of Medicine using Cas9 and guides with spacer sequences AGTGGGAAATGCTTCGAGTT, CTCAGATACTATGTACATTT, TAGTATCTGAGTAAAGATTA, and TGTGGTGTTCCTGACATAAC that targeted exon(s) ENSMUSE00000949727.2. This resulted in deletion(s) of 1243 bp (location: Chr5:77462777-77464019; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Polr2b Mutation: 60 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 5 reference(s)