Gt(ROSA)26Sor^{tm1.1(HEXB*R505Q)Clk}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(HEXB*R505Q)Clk}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Lorne A Clarke
• MGI ID: MGI:6260149
• Synonyms: hHEXB R505Q
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(HEXB*R505Q)Clk} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Nucleotide substitutions
• Gt(ROSA)26Sor^{tm1.1(HEXB*R505Q)Clk} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  HEXB (3074)

  Hexb	(MGI:96074)

  contains R505Q

• Mutation details: The human HEXB (hexosaminidase B) gene and human growth hormone polyA sequences followed by a loxP-flanked neomycin cassette are inserted into intron 1 of Gt(ROSA)26Sor placing HEXB expression under the control of the ROSA26 promoter. HEXB is modified by site-directed mutagenesis to harbor an arginine to glutamine mutation at residue 505 (R505Q). Cre-mediated recombination removed the floxed neo cassette. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1084 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)