Polr3b^em7(IMPC)Tcp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Polr3b^em7(IMPC)Tcp
• Name: polymerase (RNA) III (DNA directed) polypeptide B; endonuclease-mediated mutation 7, The Centre for Phenogenomics
• MGI ID: MGI:6257593
• Gene: Polr3b Location: Chr10:84458156-84563042 bp, + strand Genetic Position: Chr10, 41.58 cM, cytoband C1
• Alliance: Polr3b^em7(IMPC)Tcp page
• IMPC: Polr3b gene page

Mutation origin

• Strain of Origin: C57BL/6NCrl
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: This allele from project TCPR0237 was generated at the Toronto Centre for Phenogenomics by injecting Cas9 D10A mRNA and a single guide RNA with the spacer sequence TCATGTCCCTCAGACGGCAC. This resulted in a 8 bp insertion TCCAGTGT at Chromosome 10 positive strand 84632456 bp (GRCm38) and is predicted to cause a frameshift mutation with early truncation. c.305_306insTCCAGTGT; p.R103Pfs*3. (J:265051)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Polr3b Mutation: 102 strains or lines available

References

• Original: J:265051 MGI and IMPC, MGI Load of Endonuclease-Mediated Alleles (CRISPR) from the International Mouse Phenotyping Consortium (IMPC). Database Release. 2018-2023
• All: 3 reference(s)