Pole^tm1Dcas
Targeted Allele Detail

Summary

• Symbol: Pole^tm1Dcas
• Name: polymerase (DNA directed), epsilon; targeted mutation 1, Diego Castrillon
• MGI ID: MGI:6257026
• Synonyms: LSL-Pole^P286R
• Gene: Pole Location: Chr5:110434185-110485319 bp, + strand Genetic Position: Chr5, 53.45 cM, cytoband E3-E5
• Alliance: Pole^tm1Dcas page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266324
• Parent Cell Line: SM1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutations: Insertion, Single point mutation
• Mutation details: A floxed puromycin with a splice acceptor, four SV40 polyadenylation sequences and splice donor was inserted upstream of exon 8. Exon 9 was modified with a C to G point mutation to induce the amino acid substitution of arginine for proline at position 286 (P286R). This mutation is present in human cancers and leads to error-prone DNA replication. (J:266324)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pole Mutation: 103 strains or lines available

References

• Original: J:266324 Li HD, et al., Polymerase-mediated ultramutagenesis in mice produces diverse cancers with high mutational load. J Clin Invest. 2018 Aug 31;128(9):4179-4191
• All: 3 reference(s)