Ighg3^tm1Aak
Targeted Allele Detail

Summary

• Symbol: Ighg3^tm1Aak
• Name: Immunoglobulin heavy constant gamma 3; targeted mutation 1, Ahmed Khamlichi
• MGI ID: MGI:6241553
• Synonyms: A150
• Gene: Ighg3 Location: Chr12:113321062-113324852 bp, - strand Genetic Position: Chr12, 62.1 cM
• Alliance: Ighg3^tm1Aak page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:266641
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Intergenic deletion, Intragenic deletion
• Mutation details: The promoter-containing exon of the Ighg3 (immunoglobulin gamma 3) heavy chain constant region gene segment and ~250 base pairs of downstream sequence were replaced by a ~5 kb segment containing a loxP-flanked neomycin resistance cassette and a pre-rearranged V(D)J region utilizing JH2, followed by germline JH3, JH4, and Emu enhancer (VHDJH2-JH3-Jh4-Emu). The floxed selection cassette was deleted by Cre recombinase.
  
  FACS analysis of single-cell suspensions of various lymphatic compartments of homozygous mutant 19 day post coitus (dpc) embryos revealed premature expression of gamma 3 immunoglobulin (IgG3) in B cells of bone marrow, spleen, Peyer's patches, peritoneum, and follicular lymph nodes and virtual absence (<1%) of immunoglobulin mu (IgM) on B cells of all lymphatic tissues, demonstrating near-complete exclusion in cis of IgM expression from the mutant allele. In bone marrow of heterozygous mice, ~25% of B cells express only IgG3, while ~11% express only IgM (from the wild-type allele), suggesting IgG3-expressing B cells outcompete those expressing IgM. (J:266641)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ighg3 Mutation: 13 strains or lines available

References

• Original: J:266641 Puget N, et al., Complete cis Exclusion upon Duplication of the Emu Enhancer at the Immunoglobulin Heavy Chain Locus. Mol Cell Biol. 2015 Jul;35(13):2231-41
• All: 2 reference(s)