Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Fxn)Dhg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Fxn)Dhg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Daniel H Geschwind
• MGI ID: MGI:6220715
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Fxn)Dhg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254962
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Inducible, Knockdown, RMCE-ready)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(H1/tetO-RNAi:Fxn)Dhg} involves 1 genes/genome features (Fxn) View all
• Mutation details: A short hairpin RNA directed against the frataxin gene was targeted into the Rosa26 locus. The shRNA sequence is under the regulation of the tetO operator and a codon optimized tet repressor (itetR) under the control of the CAG promoter. The construct was transfected into LW-4 ES cells through Recombinase Mediated Cassette Exchange (J:254962)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

Notes

LW-4 ES cells = 129SvEv

References

• Original: J:254962 Chandran V, et al., Inducible and reversible phenotypes in a novel mouse model of Friedreich's Ataxia. Elife. 2017 Dec 19;6:e30054
• All: 2 reference(s)