Pla2g6^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Pla2g6^{tm1a(EUCOMM)Wtsi}
• Name: phospholipase A2, group VI; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:6209669
• Gene: Pla2g6 Location: Chr15:79170428-79212590 bp, - strand Genetic Position: Chr15, 37.7 cM
• Alliance: Pla2g6^{tm1a(EUCOMM)Wtsi} page
• IMPC: Pla2g6 gene page

Mutation origin

• Mutant Cell Line: Pla2g6-G04
• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The Pla2g6-G04_tv cassette was inserted upstream of the critical exon 6 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 9. The critical exon 6 through 8 are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:82809)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pla2g6 Mutation: 67 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 4 reference(s)