Gabrd^em1Menn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gabrd^em1Menn
• Name: gamma-aminobutyric acid (GABA) A receptor, subunit delta; endonuclease-mediated mutation 1, Steven Mennerick
• MGI ID: MGI:6209489
• Synonyms: delta* KI
• Gene: Gabrd Location: Chr4:155469437-155482528 bp, - strand Genetic Position: Chr4, 86.17 cM
• Alliance: Gabrd^em1Menn page

Mutation origin

• Strain of Origin: (C57BL/6 x CBA)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: Codon 269 was targeted with single-stranded oligodeoxynucleotide (ssODN) donor DNAs, sgRNAs and Cas9 using CRISPR/Cas9 technology. All components were microinjected into the cytoplasm and pronuclei of fertilized eggs. The resulting p.T269Y (p.Thr269Tyr) mutation renders the receptor picrotoxin (PTX) resistant without affecting basal receptor function. All founder mice for this allele harbored an additional mutation in intron 7, 15 nt upstream of the targeted mutation. In the chosen mouse line this is a C>A substitution that is predicted to have no effect on splicing. (J:265920)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gabrd Mutation: 28 strains or lines available

References

• Original: J:265920 Sun MY, et al., Chemogenetic Isolation Reveals Synaptic Contribution of delta GABAA Receptors in Mouse Dentate Granule Neurons. J Neurosci. 2018 Sep 19;38(38):8128-8145
• All: 3 reference(s)