P2rx7^{tm2.1(P2RX7*)Jde}
Targeted Allele Detail

Summary

• Symbol: P2rx7^{tm2.1(P2RX7*)Jde}
• Name: purinergic receptor P2X, ligand-gated ion channel, 7; targeted mutation 1.1, Jan Deussing
• MGI ID: MGI:6203043
• Synonyms: P2rx7^hQ460R
• Gene: P2rx7 Location: Chr5:122781974-122829495 bp, + strand Genetic Position: Chr5, 62.5 cM, cytoband F
• Alliance: P2rx7^{tm2.1(P2RX7*)Jde} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:251657
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The targeting vector consists from 5' to 3': a loxP site followed by the 3'-end (1.4-kb) of mouse intron 1; the murine exon 2 replaced by the human P2RX7 cDNA comprising exons 2-13 with the Gln460Arg variant; a reverse oriented selection marker flanked by frt sites which consists of a phosphoglycerate kinase (PGK) promoter driven neomycin resistance gene equipped with a bovine growth hormone (bGH) poly A signal (pA), a second loxP site and a quadruple poly A signal consisting of a bGHpA, a PGK pA, and 2 SV40 pAs. After germ-line transmission of the modified allele via homologous recombination in ES cells, offspring from male chimeras are bred. The FRT flanked neo cassette was removed by breeding to deleter-flp mice. (J:251657)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any P2rx7 Mutation: 52 strains or lines available

References

• Original: J:251657 Metzger MW, et al., Heterozygosity for the Mood Disorder-Associated Variant Gln460Arg Alters P2X7 Receptor Function and Sleep Quality. J Neurosci. 2017 Nov 29;37(48):11688-11700
• All: 1 reference(s)