Gt(ROSA)26Sor^{tm1(DDIT3)Myz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(DDIT3)Myz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Makoto Miyazaki
• MGI ID: MGI:6202519
• Synonyms: Rosa26-CHOP^loxtg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(DDIT3)Myz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:265800
• Parent Cell Line: EC7.1 (ES Cell)
• Strain of Origin: (C57BL/6J x 129X1/SvJ)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(DDIT3)Myz} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DDIT3 (1649)

  Ddit3

  (MGI:109247)

• Mutation details: The targeting vector is designed with a loxP-flanked transcriptional STOP cassette (PGK-puro-SV40 polyA), and an HA-tagged human DNA-damage inducible transcript 3 (DDIT3)-WPRE-BGH-pA cassette. This vector was inserted into the Gt(ROSA)26Sor locus via a FLP recombinase-mediated cassette exchange method. Specifically, the R26FNF3-1F1 (1F1) ES cell line used to generate the mutation was created by targeting Gt(ROSA)26Sor with a FRT- and F3-flanked PGK-neomycin selection cassette by homologous recombination in EC7.1 ES cells. (J:265800)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:265800 Shiozaki Y, et al., The CDK 9-cyclin T1 complex mediates saturated fatty acid-induced vascular calcification by inducing expression of the transcription factor CHOP. J Biol Chem. 2018 Sep 12
• All: 1 reference(s)