Col1a1^{em1(tetO-GFP/RNAi:Col1a1)Schup}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Col1a1^{em1(tetO-GFP/RNAi:Col1a1)Schup}
• Name: collagen, type I, alpha 1; endonuclease-mediated mutation 1, Detlef Schuppan
• MGI ID: MGI:6201736
• Synonyms: TGM-shRNACol1a1-4/Line1
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{em1(tetO-GFP/RNAi:Col1a1)Schup} page

Mutation origin

• Strain of Origin: (FVB/N x C57BL/6J)F1

Mutation description

• Allele Type: Endonuclease-mediated (Inducible, Knockdown, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Col1a1^{em1(tetO-GFP/RNAi:Col1a1)Schup} involves 1 genes/genome features (Col1a1) View all
• Mutation details: Talen-mediated recombination introduced a TRE sequence, an EGFP gene, an shRNA targeting Col1a1, and a poly A sequence downstream of the locus. The shRNA in this construct had the highest knockdown efficiency of those tested. (J:265449)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:265449 Molokanova O, et al., Inducible knockdown of procollagen I protects mice from liver fibrosis and leads to dysregulated matrix genes and attenuated inflammation. Matrix Biol. 2018 Mar;66:34-49
• All: 1 reference(s)