Crhr1^{tm4.1(cre)Jde}
Targeted Allele Detail

Summary

• Symbol: Crhr1^{tm4.1(cre)Jde}
• Name: corticotropin releasing hormone receptor 1; targeted mutation 4.1, Jan Deussing
• MGI ID: MGI:6201420
• Synonyms: Crhr1^iCre, Crhr1-ires-Cre
• Gene: Crhr1 Location: Chr11:104023681-104066349 bp, + strand Genetic Position: Chr11, 67.77 cM
• Alliance: Crhr1^{tm4.1(cre)Jde} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264526
• Parent Cell Line: TBV2 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crhr1^{tm4.1(cre)Jde} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crhr1 (MGI:88498)

• Mutation details: Mice expressing Cre recombinase under the control of the Crhr1 promoter were generated by using a recombinase-mediated cassette exchange (RMCE) strategy. A construct with a docking site contained (5' to 3') an attP site, loxP site, FRT site, splice acceptor, T2A cleavage site, tau/lacZ/flag reporter cassette, FRT site, neo cassette, attP site, PGK promoter and FRT site, was inserted into intron 2. The attB site-flanked Crhr1-Cre recombinase expression unit encompassed, from 5' to 3', Crhr1 intron 2 to exon 3 fused to the Crhr1 cDNA covering exons 4-13, an ires-Cre cassette with a bGH-pA, and a frt site followed by a Pgk1 polyadenylation sequence and hygromycin resistance cassette, both in inverse orientation. phiC31 integrase-mediated cassette exchange resulted in insertion of the Crhr1-Cre expression unit into the right attP site. Resulting ES cells were used to generate chimeric mice, and mouse line Crhr1^tm4Jde. The tau-lacZ reporter and hygromycin selection cassette were removed by breeding to FLPeR mice. (J:264526)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crhr1 (mouse) Summary of all recombinase alleles driven by Crhr1. Comparative matrix view of recombinase activities Alliance mouse Crhr1 gene page

Expression

In Mice Carrying this Mutation:

24 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Crhr1 Mutation: 26 strains or lines available

References

• Original: J:264526 Dedic N, et al., Chronic CRH depletion from GABAergic, long-range projection neurons in the extended amygdala reduces dopamine release and increases anxiety. Nat Neurosci. 2018 Jun;21(6):803-807
• All: 3 reference(s)