Mapkap1^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mapkap1^{tm1c(EUCOMM)Wtsi}
• Name: mitogen-activated protein kinase associated protein 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6197095
• Synonyms: Sin1^f
• Gene: Mapkap1 Location: Chr2:34296783-34514962 bp, + strand Genetic Position: Chr2, 22.84 cM
• Alliance: Mapkap1^{tm1c(EUCOMM)Wtsi} page
• IMPC: Mapkap1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264305
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34333801 of Chromosome 2 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, an SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 34334670. Exon 4 is thus flanked by loxP sites. Flp mediated recombination created a "conditional ready" (floxed) allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:264305)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapkap1 Mutation: 45 strains or lines available

References

• Original: J:264305 Arojo OA, et al., Active mTORC2 Signaling in Naive T Cells Suppresses Bone Marrow Homing by Inhibiting CXCR4 Expression. J Immunol. 2018 Aug 1;201(3):908-915
• All: 4 reference(s)