Gt(ROSA)26Sor^{tm2(CAG-Lin28b,-luc)Jhsc}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-Lin28b,-luc)Jhsc}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Johannes H Schulte
• MGI ID: MGI:6196138
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-Lin28b,-luc)Jhsc} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241988
• Parent Cell Line: ART4.12 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, RMCE-ready, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-Lin28b,-luc)Jhsc} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lin28b (MGI:3584032)

• Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the selection cassette in Gt(ROSA)26Sor^{tm1(CAG-flpe)Arte} with a 5' deleted neomycin resistance cassette, the CAG promoter, loxP flanked strong transcriptional termination site (LSL), the mouse Lin28b gene, IRES, and the firefly luciferase gene. Cre-mediated recombination can be utilized to remove the STOP cassette and get expression of the Lin28b gene. (J:241988)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:241988 Molenaar JJ, et al., LIN28B induces neuroblastoma and enhances MYCN levels via let-7 suppression. Nat Genet. 2012 Nov;44(11):1199-206
• All: 4 reference(s)