Tg(Myh6/tetO-Thbs4*)929Jmol
Transgene Detail

Summary

• Symbol: Tg(Myh6/tetO-Thbs4*)929Jmol
• Name: transgene insertion 929, Jeffery Molkentin
• MGI ID: MGI:6196024
• Synonyms: Thbs4-mCa²⁺
• Transgene: Tg(Myh6/tetO-Thbs4*)929Jmol Location: unknown
• Alliance: Tg(Myh6/tetO-Thbs4*)929Jmol page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Nucleotide substitutions
• Tg(Myh6/tetO-Thbs4*)929Jmol expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Thbs4 (MGI:1101779)	contains amino acid substitutions in calcium binding motifs

• Mutation details: Mutations in six DxDxDG calcium binding motifs (D499/501/503A, D512/514/516A, D571/573/575A, D632/634/636A, D655/557/659A, and D668/670/672A) within the T3R domains of mouse Thbs4 are under the control of a minimally active mouse alpha myosin heavy chain (Myh6) promoter fused to seven copies of the tetracycline-responsive promoter element (tetO). To make the Myh6 promoter minimally active, three GATA sites and two thyroid-like response elements are ablated. (J:264241)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:264241 Brody MJ, et al., Defective Flux of Thrombospondin-4 through the Secretory Pathway Impairs Cardiomyocyte Membrane Stability and Causes Cardiomyopathy. Mol Cell Biol. 2018 Apr 30
• All: 2 reference(s)