Gt(ROSA)26Sor^{tm1(CAG-mCherry,-EYFP*)Liiz}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-mCherry,-EYFP*)Liiz}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Li Zhang
• MGI ID: MGI:6196022
• Synonyms: STARS
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-mCherry,-EYFP*)Liiz} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:264240
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: The transgenic construct contains from 5' to 3' an attB site, a chicken beta-actin promoter (with cytomegalovirus immediate early enhancer), a Lox3172 site, mCherry reporter with a polyA tail, a 7.2 kb spacer, a loxP site, Lox3172 site, membrane bound enhanced yellow fluorescent protein (meYFP), woodchuck hepatitis virus posttranscriptional regulatory element and polyadenylation signal (WPRE-pA),a loxP site and an attB site and is integrated into the Gt(ROSA)26Sor locus using PhiC31 integrase RNA. (J:264240)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

Notes

This allele was produced in zygotes collected from mice carrying the Gt(ROSA)26Sor^tm12Luo allele, which was generated in (129X1/SvJ x 129S1/Sv)F1-Kitl⁺)-derived R1 ES cells.

References

• Original: J:264240 Ibrahim LA, et al., Sparse Labeling and Neural Tracing in Brain Circuits by STARS Strategy: Revealing Morphological Development of Type II Spiral Ganglion Neurons. Cereb Cortex. 2018 Jul 6
• All: 1 reference(s)