Nfia^tm1.1Elgaz
Targeted Allele Detail

Summary

• Symbol: Nfia^tm1.1Elgaz
• Name: nuclear factor I/A; targeted mutation 1.1, Mohamed Elgazzar
• MGI ID: MGI:6195185
• Gene: Nfia Location: Chr4:97660971-98007111 bp, + strand Genetic Position: Chr4, 45.52 cM, cytoband C4-C6
• Alliance: Nfia^tm1.1Elgaz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:244204
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: BALB/cJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The EUCOMM PGRS0001-B-F10 targeting vector containing a FRT site-flanked splice-acceptor- LacZ-loxP-Neo cassette, and loxP sites flanking exon 2, was utilized in the construction of this mutant. This selection cassette was inserted downstream of exon 2 of the targeted gene, and another loxP site was inserted upstream of exon 2. Flp-mediated recombination removed the FRT-flanked LacZ/neo cassette leaving exon 2 floxed. (J:244204)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Nfia Mutation: 28 strains or lines available

Notes

ES cell line = PRX-BALB/cJ 9

References

• Original: J:244204 McPeak MB, et al., Myeloid Cell-Specific Knockout of NFI-A Improves Sepsis Survival. Infect Immun. 2017 Apr;85(4)
• All: 3 reference(s)