Tmem119^em2Gfng
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tmem119^em2Gfng
• Name: transmembrane protein 119; endonuclease-mediated mutation 2, Guoping Feng
• MGI ID: MGI:6195080
• Synonyms: Tmem119-EGFP, Tmem119^{em2(EGFP)Gfng}
• Gene: Tmem119 Location: Chr5:113931790-113938457 bp, - strand Genetic Position: Chr5, 55.55 cM
• Alliance: Tmem119^em2Gfng page

Mutation origin

• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 endonuclease mediated genome editing is used to insert a 2A-EGFP ssDNA (encoding a viral 2A oligopeptide that mediates ribosomal skipping and an enhanced green fluorescent protein gene) with 55 bp homology arms into the stop codon of the gene. (J:280123)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Tmem119 Mutation: 25 strains or lines available

References

• Original: J:280123 Kaiser T, et al., Tmem119-EGFP and Tmem119-CreERT2 Transgenic Mice for Labeling and Manipulating Microglia. eNeuro. 2019 Jul/Aug;6(4):ENEURO.0448-18.2019
• All: 12 reference(s)