Ubap2l^tm1.2Gusa
Targeted Allele Detail

Summary

• Symbol: Ubap2l^tm1.2Gusa
• Name: ubiquitin-associated protein 2-like; targeted mutation 1.2, Guy Sauvageau
• MGI ID: MGI:6194946
• Gene: Ubap2l Location: Chr3:89907447-89959935 bp, - strand Genetic Position: Chr3, 39.19 cM, cytoband F2
• Alliance: Ubap2l^tm1.2Gusa page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:218697
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 2 and an FRT flanked pgk-neo cassette and loxP site were inserted downstream of exon 4 via homologous recombination. Flp mediated recombination removed the neo cassette. Null allele was obtained after in situ Cre recombination, using a Cre expressing plasmid. Western blot validating the absence of UBAP2L protein. (J:218697)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ubap2l Mutation: 70 strains or lines available

References

• Original: J:218697 Bordeleau ME, et al., UBAP2L is a novel BMI1-interacting protein essential for hematopoietic stem cell activity. Blood. 2014 Oct 9;124(15):2362-9
• All: 2 reference(s)