Serpine1^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Serpine1^{tm1c(EUCOMM)Hmgu}
• Name: serine (or cysteine) peptidase inhibitor, clade E, member 1; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6194589
• Synonyms: PAI-1 CKO, PAI-1^flox
• Gene: Serpine1 Location: Chr5:137090358-137101122 bp, - strand Genetic Position: Chr5, 76.16 cM
• Alliance: Serpine1^{tm1c(EUCOMM)Hmgu} page
• IMPC: Serpine1 gene page

Mutation origin

• Mutant Cell Line: HEPD0744_8_B03
• Germline Transmission: Earliest citation of germline transmission: J:248177
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at a position on Chromosome 5 upstream of exon 4 of the gene. The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 5. Flp-mediated recombination removed the FRT-flanked beta-gal/neomycin resistance cassette leaving exons 4 and 5 floxed. (J:248177)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Serpine1 Mutation: 24 strains or lines available

References

• Original: J:248177 Jiang C, et al., Serpine 1 induces alveolar type II cell senescence through activating p53-p21-Rb pathway in fibrotic lung disease. Aging Cell. 2017 Oct;16(5):1114-1124
• All: 2 reference(s)