Tg(Myh6-TFRC/OVA)#Ahlm
Transgene Detail

Summary

• Symbol: Tg(Myh6-TFRC/OVA)#Ahlm
• Name: transgene insertion, Andrew H Lichtman
• MGI ID: MGI:6192766
• Synonyms: cMy-mOVA
• Transgene: Tg(Myh6-TFRC/OVA)#Ahlm Location: unknown
• Alliance: Tg(Myh6-TFRC/OVA)#Ahlm page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Myh6-TFRC/OVA)#Ahlm expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  TFRC (7037)

  Tfrc	(MGI:98822)

  mouse promoter and N-terminal fragment of human gene fused to part of chicken ovalbumin

• Mutation details: The transgene comprises the following elements: the mouse cardiac myosin heavy chain promoter, DNA sequence coding for amino-acids 2-118 of the human transferrin receptor (includes transmembrane sequence), DNA sequence coding for amino-acids 139-387 of the chicken ovalbumin gene, and, from the pcDNA3.1V5-His6-TOPO plasmid, V5 epitope sequence, His6 tag sequence, stop codon, and bovine growth hormone polyA signal. RT-PCR, ELISA and immunofluorescence experiments confirmed exclusive expression in myocardium. The construct was microinjected into embryonic stem cells. Mice carying this transgene will express a membrane-bound chimeric protein with the ovalbumin peptide exposed extracellularly. The ovalbumin peptide includes the two sequences that are recognised by the T cell receptors expressed by transgenes Tg(TcraTcrb)1100Mjb (OT-I) and Tg(TcraTcrb)425Cbn (OT-II) respectively. (J:132693)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:132693 Grabie N, et al., IL-12 is required for differentiation of pathogenic CD8+ T cell effectors that cause myocarditis. J Clin Invest. 2003 Mar;111(5):671-80
• All: 3 reference(s)