Tg(TCROb.1A12)8Dma
Transgene Detail

Summary

• Symbol: Tg(TCROb.1A12)8Dma
• Name: transgene insertion 8, Daniel M Altmann
• MGI ID: MGI:6192077
• Synonyms: Line 8
• Transgene: Tg(TCROb.1A12)8Dma Location: unknown
• Alliance: Tg(TCROb.1A12)8Dma page

Transgene origin

• Strain of Origin: (CBA x C57BL/6)F2

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: PCR primers with flanking restriction sites were designed to amplify the rearranged Valpha3.1Jalpha40 and Vbeta2.1Jbeta2.1 segments from genomic DNA of the Ob1A12 T cell clone. These DNA segments were then subcloned into murine TCR-specific regulatory elements containing genomic TCR expression cassettes pTalphaCass and pTbetaCass, respectively, and microinjected into fertilized oocytes. Mice with this transgene express human anti-MBP (myelin basic protein) 85-99/DR15 T cell receptors. (J:90756)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:90756 Ellmerich S, et al., Disease-related epitope spread in a humanized T cell receptor transgenic model of multiple sclerosis. Eur J Immunol. 2004 Jul;34(7):1839-48
• All: 2 reference(s)