Stxbp5l^tm1.1Aljg
Targeted Allele Detail

Summary

• Symbol: Stxbp5l^tm1.1Aljg
• Name: syntaxin binding protein 5-like; targeted mutation 1.1, Alexander J Groffen
• MGI ID: MGI:6191659
• Synonyms: Tom2^KO
• Gene: Stxbp5l Location: Chr16:36935304-37205324 bp, - strand Genetic Position: Chr16, 26.32 cM
• Alliance: Stxbp5l^tm1.1Aljg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263474
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The second coding exon was flanked by loxP sites. An frt-flanked neomycin selection cassette was inserted between the loxP site and the 5 end of exon 2. Exon 2 and the neomycin selection cassette was removed by Cre-mediated recombination. Quantitative PCR analysis of homozygous mutant mouse mRNA in cortex and spinal cord confirmed the absence of exon 2 containing mRNA. Western blot analysis of brain extracts of homozygous mice indicated that no detectable encoded protein was present. (J:263474)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Stxbp5l Mutation: 60 strains or lines available

References

• Original: J:263474 Geerts CJ, et al., Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates. Brain Struct Funct. 2015 Jul;220(4):1971-82
• All: 1 reference(s)