Mir33^tm2.1Koho
Targeted Allele Detail

Summary

• Symbol: Mir33^tm2.1Koho
• Name: microRNA 33; targeted mutation 2.1, Koh Ono
• MGI ID: MGI:6188638
• Synonyms: miR-33^flox
• Gene: Mir33 Location: Chr15:82082323-82082391 bp, + strand Genetic Position: Chr15, 38.54 cM
• Alliance: Mir33^tm2.1Koho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:262024
• Parent Cell Line: CMTI-2 (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A loxP site was inserted 40 bp upstream of the coding region of the target gene, and a second loxP site downstream. An FRT site flanked neomycin resistance gene cassette was inserted between the target gene and the downstream loxP site. The neo cassette was subsequently removed through flp-mediated recombination, leaving a conditional-ready allele with the gene floxed. (J:262024)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir33 Mutation: 1 strain or line available

References

• Original: J:262024 Nishiga M, et al., MicroRNA-33 Controls Adaptive Fibrotic Response in the Remodeling Heart by Preserving Lipid Raft Cholesterol. Circ Res. 2017 Mar 3;120(5):835-847
• All: 1 reference(s)