C1qtnf12^tm1Gww
Targeted Allele Detail

Summary

• Symbol: C1qtnf12^tm1Gww
• Name: C1q and tumor necrosis factor related 12; targeted mutation 1, G William Wong
• MGI ID: MGI:6164618
• Synonyms: Ctrp12^-
• Gene: C1qtnf12 Location: Chr4:156046775-156051086 bp, + strand Genetic Position: Chr4, 87.66 cM
• Alliance: C1qtnf12^tm1Gww page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242627
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An FRT flanked gene trap cassette composed of an FRT site, a splice acceptor, and a promoterless lacZ reporter followed by a polyadenylation signal, a loxP site, a promoter-driven neomycin resistance gene, a second polyadenylation signal and a second FRT site, was inserted via homologous recombination between exons 1 and 2, disrupting the gene. Additional loxP sites were inserted flanking exons 2 through 8 rendering this a conditional-ready allele. Splicing of exon 1 to the lacZ reporter containing the polyadenylation signal on the 3 end yields a severely truncated polypeptide if translated. Quantitative PCR analysis on cDNA isolated from gonadal and subcutaneous (inguinal) white adipose depots and kidney showed a ~50% reduction of mRNA levels in heterozygous mutant mice. (J:242627)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C1qtnf12 Mutation: 20 strains or lines available

References

• Original: J:242627 Tan SY, et al., Partial deficiency of CTRP12 alters hepatic lipid metabolism. Physiol Genomics. 2016 Dec 01;48(12):936-949
• All: 2 reference(s)