Cntd1^{tm1e.1(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cntd1^{tm1e.1(KOMP)Wtsi}
• Name: cyclin N-terminal domain containing 1; targeted mutation 1e.1, Wellcome Trust Sanger Institute
• MGI ID: MGI:6161819
• Synonyms: Cntd1^GT
• Gene: Cntd1 Location: Chr11:101170029-101179527 bp, + strand Genetic Position: Chr11, 64.56 cM
• Alliance: Cntd1^{tm1e.1(KOMP)Wtsi} page
• IMPC: Cntd1 gene page

Mutation origin

• Mutant Cell Line: EPD0190_3_E03
• Germline Transmission: Earliest citation of germline transmission: J:215796
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 101173775 of Chromosome 11 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. Insertion of this cassette creates a reporter knockout mouse. Cre expression removed the neomycin selection cassette. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:215796)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cntd1 Mutation: 13 strains or lines available

References

• Original: J:215796 Holloway JK, et al., Mammalian CNTD1 is critical for meiotic crossover maturation and deselection of excess precrossover sites. J Cell Biol. 2014 Jun 9;205(5):633-41
• All: 1 reference(s)