Hprt1^{tm1(tetO-EGFP/MIR125B1)Jlu}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm1(tetO-EGFP/MIR125B1)Jlu}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 1, Jun Lu
• MGI ID: MGI:6161212
• Synonyms: i125b
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm1(tetO-EGFP/MIR125B1)Jlu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240427
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Hprt1^{tm1(tetO-EGFP/MIR125B1)Jlu} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MIR125B1 (406911)

  Mir125b-1

  (MGI:2676810)

  inducible EGFP/MIR125B1 fusion

• Mutation details: The targeting vector was designed with a human MIR125B1 cDNA inserted between 2 splicing sites in the 3' untranslated region of an EGFP sequence and followed by a polyA signal. The targeting vector was then used to replace the Cre ORF in a mutant embryonic stem cell line (see note) and placed under the control of the tet response promoter (TRE). The construct was inserted into a cassette exchange locus upstream of the Hprt gene using Inducible Cassette Exchange (ICE) recombination. (J:240427)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1274 strains or lines available

Notes

ES cell line = a mutant A17 line (A2Lox.cre) that carries a doxycycline-inducible Cre recombinase ORF flanked by loxP and lox2272 sites, upstream of a non-functional neomycin resistance (neo) gene.

References

• Original: J:240427 Liu J, et al., miR-125b promotes MLL-AF9-driven murine acute myeloid leukemia involving a VEGFA-mediated non-cell-intrinsic mechanism. Blood. 2017 Mar 16;129(11):1491-1502
• All: 1 reference(s)