Pofut1^tm2.1Pst
Targeted Allele Detail

Summary

• Symbol: Pofut1^tm2.1Pst
• Name: protein O-fucosyltransferase 1; targeted mutation 2.1, Pamela Stanley
• MGI ID: MGI:6160874
• Gene: Pofut1 Location: Chr2:153083453-153112167 bp, + strand Genetic Position: Chr2, 75.41 cM, cytoband H2
• Alliance: Pofut1^tm2.1Pst page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: WW6 (ES Cell)
• Strain of Origin: STOCK 129/Sv and C57BL/6J and SJL

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The targeting vector was designed to insert a single loxP site upstream of exon 2, and a loxP-flanked neomycin resistance (neo) cassette downstream of exon 2.Correctly targeted ES cells were transiently transfected with a pMC1 Cre Recombinase expression plasmid to delete the neo cassette. (J:83299)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pofut1 Mutation: 22 strains or lines available

Notes

The version of this allele that contains the neomycin selection cassette, Pofut1^tm2Pst, has been analyzed.

References

• Original: J:83299 Shi S, et al., Protein O-fucosyltransferase 1 is an essential component of Notch signaling pathways. Proc Natl Acad Sci U S A. 2003 Apr 29;100(9):5234-9
• All: 8 reference(s)