Gt(ROSA)26Sor^em5Mvw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^em5Mvw
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 5, Michael Wiles
• MGI ID: MGI:6160836
• Synonyms: NSG.Bxb1v2, NSG.RosaBxb-GT/GA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^em5Mvw page

Mutation origin

• Strain of Origin: NOD.Cg-Prkdc^scid Il2rg^tm1Wjl/SzJ

Mutation description

• Allele Type: Endonuclease-mediated (RMCE-ready)
• Mutation: Insertion
• Mutation details: CRISPR/cas9 genome editing used guide RNAs to target intron 1 of the Gt(ROSA)26Sor locus. Oligonucleotide donor DNAs were created encoding a duel landing pad flanked by the heterologous attP attachment sites - specifically a 48 bp Bxb1 attP-GT sequence, a 240 bp spacer of native Gt(ROSA)26Sor sequence and a 48 bp Bxb1 attP-GA sequence. (J:322700)
• Inheritance: Not Specified

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 944 strains or lines available

References

• Original: J:322700 Low BE, et al., Efficient targeted transgenesis of large donor DNA into multiple mouse genetic backgrounds using bacteriophage Bxb1 integrase. Sci Rep. 2022 Mar 31;12(1):5424
• All: 1 reference(s)