Tg(APOB*Q2153L)#Boren
Transgene Detail

Summary

• Symbol: Tg(APOB*Q2153L)#Boren
• Name: transgene insertion, Jan Boren
• MGI ID: MGI:6160100
• Synonyms: apo-B100 Leu-Leu, Tg(APOB100)
• Transgene: Tg(APOB*Q2153L)#Boren Location: unknown
• Alliance: Tg(APOB*Q2153L)#Boren page

Transgene origin

• Strain of Origin: (C57BL/6 x SJL)

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence, Modified isoform(s))
• Mutation: Insertion
• Tg(APOB*Q2153L)#Boren expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOB (338)

  Apob	(MGI:88052)

  expresses only APOB-100, and not APOB-48, isoform

• Mutation details: The transgene contains the full-length human apolipoprotein B gene with a CAA to CTA point mutation in codon 2153, which results in the replacement of glutamine by leucine (Q2153L). Loss of the CAA prevents its conversion to the stop codon UAA by a liver mRNA editing mechanism, abrogating production of apo-B48 and increasing the yield of the full-length translation product, apo-B100. This mutation is called "apo-B100 Leu-Leu" in the literature because conversion by mRNA editing of the C in the mutant leucine-encoding CTA codon to U would yield UUA, which also encodes leucine. (J:262089)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:262089 Boren J, et al., Identification of the low density lipoprotein receptor-binding site in apolipoprotein B100 and the modulation of its binding activity by the carboxyl terminus in familial defective apo-B100. J Clin Invest. 1998 Mar 1;101(5):1084-93
• All: 5 reference(s)