Gt(ROSA)26Sortm1(CAG-KDM1A/EGFP)Rsd
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1(CAG-KDM1A/EGFP)Rsd |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Michael G Rosenfeld |
MGI ID: |
MGI:6160075 |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1(CAG-KDM1A/EGFP)Rsd page
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Allele Type: |
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Targeted (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence, Modified isoform(s), Reporter) |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm1(CAG-KDM1A/EGFP)Rsd expresses
1 gene
Knock-in expresses:
Organism |
Expressed Gene |
Homolog in Mouse |
Note |
human |
KDM1A (23028) |
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conditional: expressed only after removal of floxed STOP cassette; human gene is fused with EGFP |
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Mutation details: The targeting cassette contains CAG-loxP-(PGK-neomycin - 4x polyA) Stop-loxP-biotin ligase recognition peptide (BLRP)-3x FLAG-LSD1 WT-IRES2-EGFP-4x polyA. The LSD1 VN (KDM1A) insertion expresses a FLAG-tagged full-length human KDM1A cDNA, which encodes an 852 amino acid isoform of the gene that includes a 60 nucleotide exon in intron 2 (E2a) as well as an EGFP fluorescent reporter.
(J:101977)
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Original: |
J:225803 Wang J, et al., LSD1n is an H4K20 demethylase regulating memory formation via transcriptional elongation control. Nat Neurosci. 2015 Sep;18(9):1256-64 |
All: |
2 reference(s) |
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