Tg(tetO-DMPK*)A2352Coop
Transgene Detail

Summary

• Symbol: Tg(tetO-DMPK*)A2352Coop
• Name: transgene insertion A2352, Thomas A Cooper
• MGI ID: MGI:6158679
• Synonyms: TREDT960I
• Transgene: Tg(tetO-DMPK*)A2352Coop Location: unknown Genetic Position: Chr1, Syntenic
• Alliance: Tg(tetO-DMPK*)A2352Coop page

Transgene origin

• Strain of Origin: FVB/NJ

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-DMPK*)A2352Coop expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DMPK (1760)

  Dmpk	(MGI:94906)

  Contains 960 interrupted CTG repeats

• Mutation details: The transgene contains a human genomic segment containing exons 11-15 of the DMPK gene with 960 interrupted CTG repeats (CUG>960<) in exon 15 (at the site of the endogenous CTG repeats). These interrupted repeats are generated to contain 20 CTGs separated by five nucleotide spacers. The construct also contains 307 bp of the DMPK 3' flanking region containing the natural polyadenylation signals, and two copies of the cHS4 insulator flanking the 5' and 3' ends of the expression construct to prevent effects of the chromosomal insertion site. This mutant DMPK gene is under the direction of the tetO (tet-responsive element) promoter. The transgene integrated in chromosome 1 (9,622,984-9,660,005) is accompanied by a 37 kb genomic duplication. The integration site is located in the intronic region of 2610203C22Rik. It was also noted at that time that five nucleotide changes are in the transgene compared to the human DMPK sequence. Four of these changes (in intron 11 [CCCTTGGCAgCCCCTCTCGT], intron 14 [CACCCGCCCCgTGCCACCCG], and exon 15 [TTGCCAAACCtGCTTTTTCG and CCCAGCCGGCcCCGCCCGCT]) have no known effect on the Tg. One change, in exon 15 [CCTCGGTATcTATTGT], may affect the binding site for an antisense oligo. Similar to the human gene, it was discovered that two exons in the transgene undergo alternative splicing. (J:264569)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available

References

• Original: J:264569 Morriss GR, et al., Mechanisms of skeletal muscle wasting in a mouse model for myotonic dystrophy type 1. Hum Mol Genet. 2018 Aug 15;27(16):2789-2804
• All: 3 reference(s)