Gt(ROSA)26Sor^{tm1.1(CAG-tdTomato/GCaMP6f)Mdcah}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-tdTomato/GCaMP6f)Mdcah}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Michael Cahalan
• MGI ID: MGI:6157515
• Synonyms: Gt(ROSA)26Sor^{pCAG-FRT-LSL-Salsa6f-WPRE-bGHpA-AttB/P}, LSL-Salsa6f
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-tdTomato/GCaMP6f)Mdcah} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254963
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains (5' to 3'): CAG (cytomegalovirus early enhancer/chicken beta-actin) promoter, an FRT site, loxP-STOP-loxP cassette, Salsa6f (see below), WPRE, bGHpA, AttB site, an FRT site, neomycin selection cassette, AttP site (using the Addgene Plasmid #34883); inserted into the Gt(ROSA)26Sor locus. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence. Salsa6f contains tdTomato linked to GCaMP6f by a V5 epitope tag. (J:254963)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:254963 Dong TX, et al., T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse. Elife. 2017 Dec 14;6:e32417
• All: 3 reference(s)