Tg(tetO-AstA-R1)2229Kent
Transgene Detail

Summary

• Symbol: Tg(tetO-AstA-R1)2229Kent
• Name: transgene insertion 2229, Clifford G Kentros
• MGI ID: MGI:6157431
• Synonyms: AlstR/tetO
• Transgene: Tg(tetO-AstA-R1)2229Kent Location: unknown
• Alliance: Tg(tetO-AstA-R1)2229Kent page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Inserted expressed sequence)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Mutation details: The transgenic construct consists of the tetO, tetracycline-responsive regulatory element (from pTRE-tight), cDNA encoding the Drosophila AstA-R1, or AlstR, allatostatin A receptor 1, gene and a SV40 polyadenylation sequence. The TRE promoter used here is Tet-responsive Ptight; containing a modified Tet response element composed of seven direct 36 bp repeats (each containing the 19 bp tet operator sequence) and a minimal human cytomegalovirus promoter, which lacks the enhancer that is part of the complete CMV promoter. Consequently, Ptight is silent in the absence of TetR or rTetR binding to tetO. (J:261666)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:261666 Wehr M, et al., Transgenic silencing of neurons in the mammalian brain by expression of the allatostatin receptor (AlstR). J Neurophysiol. 2009 Oct;102(4):2554-62
• All: 2 reference(s)