Tg(Igk-mNeonGreen/mRuby2)50.28Mnz
Transgene Detail

Summary

• Symbol: Tg(Igk-mNeonGreen/mRuby2)50.28Mnz
• Name: transgene insertion 50.28, Michel C Nussenzweig
• MGI ID: MGI:6156660
• Synonyms: Igkappa^INDIA
• Transgene: Tg(Igk-mNeonGreen/mRuby2)50.28Mnz Location: unknown
• Alliance: Tg(Igk-mNeonGreen/mRuby2)50.28Mnz page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The reporter cDNA is introduced to mouse Igk regulatory elements: Vkappa21E promoter-noncoding leader (L) exon-Vkappa exon-mNeonGreen/mRuby2 (INDIA) cDNA-polyA-Jkappa5 intronic sequence with enhancer (ie) and 3' regulatory region (rr). The reporter consists of an optimized fluorescence (Forster) resonance energy transfer (FRET) pair: N-terminally Myc-tagged mNeonGreen and mRuby2, linked by an 18 amino acid peptide containing an aCasp3-cleavage site (DEVDG). When aCasp3 (Casp3, caspase 3; a marker for apoptosis) cleaves the linker, mNeonGreen and mRuby2 are separated, resulting in FRET loss and increased emission from mNeonGreen. Non-apoptotic cells retaining uncleaved mNeonGreen/mRuby2 demonstrate more yellow fluorescence indicating a lower ratio of mNeonGreen/FRET fluorescence emission. (J:261534)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:261534 Mayer CT, et al., The microanatomic segregation of selection by apoptosis in the germinal center. Science. 2017 Oct 13;358(6360)
• All: 1 reference(s)