Scn4b^tm1.2Geh
Targeted Allele Detail

Summary

• Symbol: Scn4b^tm1.2Geh
• Name: sodium channel, type IV, beta; targeted mutation 1.2, Gregg E Homanics
• MGI ID: MGI:6155711
• Synonyms: Scn4b KO
• Gene: Scn4b Location: Chr9:45049922-45065453 bp, + strand Genetic Position: Chr9, 24.84 cM
• Alliance: Scn4b^tm1.2Geh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:261348
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A targeting vector was designed to insert a loxP site, followed by a FRT-flanked neomycin resistance (neo) cassette, upstream of exon 2, and a second loxP site downstream of exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exon 2 floxed. Cre-mediated recombination removed exon 2, which is predicted to cause a frameshift mutation resulting in a truncated product. Western blot analysis confirmed the absence of the 37 kD protein. (J:261348)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Scn4b Mutation: 19 strains or lines available

References

• Original: J:261348 Ji X, et al., The Sodium Channel beta4 Auxiliary Subunit Selectively Controls Long-Term Depression in Core Nucleus Accumbens Medium Spiny Neurons. Front Cell Neurosci. 2017;11:17
• All: 2 reference(s)