Ncf2^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ncf2^{tm1b(EUCOMM)Wtsi}
• Name: neutrophil cytosolic factor 2; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6153187
• Synonyms: Ncf2^-
• Gene: Ncf2 Location: Chr1:152675945-152712742 bp, + strand Genetic Position: Chr1, 65.05 cM
• Alliance: Ncf2^{tm1b(EUCOMM)Wtsi} page
• IMPC: Ncf2 gene page

Mutation origin

• Mutant Cell Line: EPD0240_5_F04
• Germline Transmission: Earliest citation of germline transmission: J:260777
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette containing a FRT-En2 splice acceptor (SA)-IRES-lacZ-SV40 polyadenylation signal (pA)-loxP-human Beta-actin promoter-neoR-SV40 polyadenylation signal (pA)-FRT-loxP sequence was inserted upstream of exon 3 and an additional loxP site downstream of exon 3 (at position 152817361). Cre-mediated recombination removed the floxed neo cassette and exon 3. No gene product (protein) is detected by western blot analysis of neutrophil cell lysates from homozygotes. (J:260777)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ncf2 Mutation: 37 strains or lines available

References

• Original: J:260777 Jacob CO, et al., Haploinsufficiency of NADPH Oxidase Subunit Neutrophil Cytosolic Factor 2 Is Sufficient to Accelerate Full-Blown Lupus in NZM 2328 Mice. Arthritis Rheumatol. 2017 Aug;69(8):1647-1660
• All: 4 reference(s)