Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy
Transgene Detail
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Symbol: |
Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy |
Name: |
transgene insertion 10, Wei Yang |
MGI ID: |
MGI:6150883 |
Synonyms: |
CAG-SUMO |
Transgene: |
Tg(CAG-EGFP,-Sumo)10Weiy Location: unknown
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Alliance: |
Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy page
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Transgene Type: |
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Transgenic (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter) |
Mutation: |
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Insertion
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Tg(CAG-EGFP,-Sumo1,-Sumo2,-Sumo3,-mCherry)10Weiy expresses
3 genes
Transgene expresses:
Organism |
Expressed Gene |
Note |
mouse |
Sumo1 (MGI:1197010) |
includes a histidine tag |
mouse |
Sumo2 (MGI:2158813) |
includes a hemagglutin tag |
mouse |
Sumo3 (MGI:1336201) |
includes a FLAG tag |
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Mutation details: The transgene was designed to have (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP site, EGFP, a transcriptional/translational STOP cassette (containing a PGK-neo), a second loxP site, a mouse cDNA sequence encoding precursor SUMO1 with N-terminal histidine tag (His-SUMO1), a viral 2A oligopeptide sequence (P2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO2 with N-terminal hemagglutinin tag (HA-SUMO2), a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping), a mouse cDNA sequence encoding precursor SUMO3 with N-terminal FLAG tag (FLAG-SUMO3), a viral 2A oligopeptide sequence (F2A; mediates ribosomal skipping), the monomeric DsRed fluorescent protein variant mCherry gene and a bovine growth hormone polyadenylation signal. Eight founder lines were created, founder line 10 exhibits ubiquitous expression of GFP.
(J:260338)
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Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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Original: |
J:260338 Yang W, et al., Small ubiquitin-like modifier 3-modified proteome regulated by brain ischemia in novel small ubiquitin-like modifier transgenic mice: putative protective proteins/pathways. Stroke. 2014 Apr;45(4):1115-22 |
All: |
1 reference(s) |
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