Zswim6^tm1.2Itl
Targeted Allele Detail

Summary

• Symbol: Zswim6^tm1.2Itl
• Name: zinc finger SWIM-type containing 6; targeted mutation 1.2, inGenious Targeting Laboratory
• MGI ID: MGI:6149981
• Gene: Zswim6 Location: Chr13:107861152-108026598 bp, - strand Genetic Position: Chr13, 59.39 cM
• Alliance: Zswim6^tm1.2Itl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259070
• Parent Cell Line: iTL BA1 (ES Cell)
• Strain of Origin: (C57BL/6NTac x 129S6/SvEvTac)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A FRT-LoxP-Neomycin-FRT-LoxP selection cassette was inserted downstream of exon 4 and the third single LoxP site was inserted upstream of exon 4. The region flanked by the second and third LoxP sites is approximately 569 bp. Exon 4 encodes amino acids 337-386 and deletion of this sequence results in the creation of a premature stop codon. The neomycin selection cassette was removed via FLP-mediated recombination, leaving loxP sites flanking exon 4. Exon 4 was susequently removed via Cre-mediated recombination. In situ hybridization and RT-PCR analysis confirmed that this allele lacks expression of exon 4. qPCR analysis revealed that the medial ganglionic eminence of homozygous mutants expresses transcript downstream of the deleted region at ~35% of control levels at E13.5. (J:259070)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zswim6 Mutation: 60 strains or lines available

References

• Original: J:259070 Tischfield DJ, et al., Loss of the neurodevelopmental gene Zswim6 alters striatal morphology and motor regulation. Neurobiol Dis. 2017 Jul;103:174-183
• All: 1 reference(s)