Spi1^tm1.1Mbu
Targeted Allele Detail

Summary

• Symbol: Spi1^tm1.1Mbu
• Name: Spi-1 proto-oncogene; targeted mutation 1.1, Meinrad Busslinger
• MGI ID: MGI:6149548
• Synonyms: Spi1^Bio
• Gene: Spi1 Location: Chr2:90912750-90946104 bp, + strand Genetic Position: Chr2, 50.44 cM, cytoband E3
• Alliance: Spi1^tm1.1Mbu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:259465
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Epitope tag)
• Mutation: Insertion
• Mutation details: A PreScission cleavage site-FLAG-V5-TEV cleavage site-biotin tag construct, an FRT flanked IRES-CD2 reporter construct and a loxP flanked pgk-neo cassette were inserted in the end of exon 5 via homologous recombination. Recombinase mediated recombination removed the reporter and neo cassettes. (J:259465)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spi1 Mutation: 31 strains or lines available

References

• Original: J:259465 Minnich M, et al., Multifunctional role of the transcription factor Blimp-1 in coordinating plasma cell differentiation. Nat Immunol. 2016 Mar;17(3):331-43
• All: 1 reference(s)