Tg(GFAP-CHRM3*)6Kdmc
Transgene Detail

Summary

• Symbol: Tg(GFAP-CHRM3*)6Kdmc
• Name: transgene insertion 6, Kevin D McCarthy
• MGI ID: MGI:6148045
• Synonyms: GFAP-DREADD 3, GFAP-Gq-DREADD, GFAP-hM3Dq
• Transgene: Tg(GFAP-CHRM3*)6Kdmc Location: unknown
• Alliance: Tg(GFAP-CHRM3*)6Kdmc page

Transgene origin

• Strain of Origin: C3H/HeJ x C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(GFAP-CHRM3*)6Kdmc expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CHRM3 (1131)

  Chrm3

  (MGI:88398)

  Expresses a mutation hM3Dq

• Mutation details: This transgene expresses a modified human CHRM3 sequence (DREADD or hM3dq) under the control of a human GFAP promoter.
  The hM3Dq ("DREADD") sequence is a Gq-coupled human M3 muscarinic DREADD (designer receptor exclusively activated by designer drug). To create the hM3Dq sequence, the wildtype human muscarinic 3 receptor (CHRM3) sequence was modified via site-directed mutagenesis to harbor two amino acid substitutions (Y149C<3.33>/A239G<5.46>) that abolish receptor affinity for the native ligand, acetylcholine (ACh), but allow receptor binding and subsequent activation by the small drug-like molecule clozapine-N-oxide (CNO). (J:215844)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:215844 Agulhon C, et al., Modulation of the autonomic nervous system and behaviour by acute glial cell Gq protein-coupled receptor activation in vivo. J Physiol. 2013 Nov 15;591(Pt 22):5599-609
• All: 7 reference(s)