Map2k1^{tm1(Map2k1,Map2k2)Chrn}
Targeted Allele Detail

Summary

• Symbol: Map2k1^{tm1(Map2k1,Map2k2)Chrn}
• Name: mitogen-activated protein kinase kinase 1; targeted mutation 1, M Jean Charron
• MGI ID: MGI:6148035
• Synonyms: Mek1^1neo2
• Gene: Map2k1 Location: Chr9:64093066-64160887 bp, - strand Genetic Position: Chr9, 34.55 cM
• Alliance: Map2k1^{tm1(Map2k1,Map2k2)Chrn} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:258488
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence, Null/knockout)
• Mutation: Insertion
• Map2k1^{tm1(Map2k1,Map2k2)Chrn} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Map2k1 (MGI:1346866)

• Mutation details: A cDNA of Map2k1 (tagged with Myc and flanked by loxP sites), an frt-flanked neomycin selection cassette, and a cDNA of Map2k2 were inserted into the first exon of Mek1. Western blot analysis showed that both endogenous Map2k1 protein and the Myc-tagged Map2k1 protein are expressed in heterozygous embryonic stem (ES) cells. (J:258488)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map2k1 Mutation: 94 strains or lines available

References

• Original: J:258488 Aoidi R, et al., Functional redundancy of the kinases MEK1 and MEK2: Rescue of the Mek1 mutant phenotype by Mek2 knock-in reveals a protein threshold effect. Sci Signal. 2016 Jan 26;9(412):ra9
• All: 1 reference(s)