Tg(Eno2-MMP9*)EPfzr
Transgene Detail

Summary

• Symbol: Tg(Eno2-MMP9*)EPfzr
• Name: transgene insertion E, Jeffrey Stock
• MGI ID: MGI:6144248
• Synonyms: G100L-Tg-LineE
• Transgene: Tg(Eno2-MMP9*)EPfzr Location: unknown
• Alliance: Tg(Eno2-MMP9*)EPfzr page

Transgene origin

• Strain of Origin: FVB/N

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(Eno2-MMP9*)EPfzr expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  MMP9 (4318)

  Mmp9	(MGI:97011)

  Expresses an mutation G100L

• Mutation details: The transgenic construct contains a rat enolase 2, gamma neuronal (Eno2) promoter, containing a random filler sequence and an SV40 polyadenylation signal, directing expression of human matrix metallopeptidase 9 (MMP9) cDNA. The MMP9 gene was modified to harbor a glycine to leucine mutation at residue 100 (MMP9*G100L), in the cysteine switch autoinhibitor region, rendering the enzyme constitutively active. Of the five lines, founder line exhibits the highest transgene activity. (J:258077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:258077 Fisher KE, et al., Engineering autoactivating forms of matrix metalloproteinase-9 and expression of the active enzyme in cultured cells and transgenic mouse brain. Biochemistry. 2002 Jul 2;41(26):8289-97
• All: 1 reference(s)