Cnnm4^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cnnm4^{tm1c(EUCOMM)Wtsi}
• Name: cyclin M4; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6144059
• Synonyms: Cnnm4^flox
• Gene: Cnnm4 Location: Chr1:36510701-36547845 bp, + strand Genetic Position: Chr1, 15.28 cM, cytoband B
• Alliance: Cnnm4^{tm1c(EUCOMM)Wtsi} page
• IMPC: Cnnm4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246429
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 36497561 of Chromosome 1 upstream of the critical exon(s) (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 36500237. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:246429)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cnnm4 Mutation: 18 strains or lines available

References

• Original: J:246429 Yamazaki D, et al., The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction. J Cell Sci. 2016 May 01;129(9):1940-9
• All: 1 reference(s)