Trp63^tm2.1Cmis
Targeted Allele Detail

Summary

• Symbol: Trp63^tm2.1Cmis
• Name: transformation related protein 63; targeted mutation 2.1, Caterina Missero
• MGI ID: MGI:6140146
• Synonyms: p63^L514Fflox
• Gene: Trp63 Location: Chr16:25502513-25710842 bp, + strand Genetic Position: Chr16, 17.37 cM
• Alliance: Trp63^tm2.1Cmis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:257774
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag)
• Mutations: Insertion, Single point mutation
• Mutation details: The targeting construct introduced into the locus a loxP site, a cDNA encoding wild-type exon 13-14 with a polyadenylation sequence, an FRT-flanked neomycin resistance cassette with a 3' loxP site, a modified exon 13 with a nucleotide substitution that results in the amino acid substitution of phenylalanine for leucine at position 514, and a 3xFLAG tag on exon 14. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination is required to achieve expression of the modified exon 13. (J:257774)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Trp63 Mutation: 58 strains or lines available

References

• Original: J:257774 Russo C, et al., Protein aggregation of the p63 transcription factor underlies severe skin fragility in AEC syndrome. Proc Natl Acad Sci U S A. 2018 Jan 30;115(5):E906-E915
• All: 2 reference(s)